PROBLEMS WITH OTHER VIBRATOMES
There are many different types of tissue slicers on the market, but they don’t operate the way that the Compresstome® does. Other market vibratomes will have a flat stage where you glue your tissue sample, with a cutting blade that approaches horizontally. However, the blade can easily push your tissue sample and squish, tilt, and compress it—all of these effects may damage cells and tissue architecture.
BENEFITS OF SLIGHT COMPRESSION FOR TISSUE SLICING
When using the Compresstome®, you will embed your tissue in agarose inside a specimen tube. The open end of the specimen tube has a slightly tapered edge. As tissue and agarose cross the edge of the tube, it gets slightly compressed. This compression helps stabilize the tissue during sectioning, and allows the Compresstome® to section at speeds up to 5x faster than other market vibrating microtomes. Overall, the Compresstome® provides a significant improvement on the health and longevity of live slices, and virtually eliminates chattermarks in fixed tissue slices.
Don’t worry–The agarose embedding does not decrease cell viability. Rather, it enhances cell survival because of tissue stability. The agarose solution is quickly cooled down with a chilling block during sample preparation. With the Compresstome®, your tissue can be sectioned faster because of the agarose-induced stability. As a result, cells are never without oxygenation for a long time, and the added stability of the compression significantly reduces any blade damage.
For almost 20 years, Precisionary Instruments has specialized in designing and manufacturing tissue slicers. With our patented technology, we are devoted to helping you obtain the highest quality tissue slices for research and clinical needs.
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