PINNACLE’S SLEEP DEPRIVATION SYSTEM is designed to gently restrict sleep without unnecessary exercise in rodents. This is achieved by a rotating bar placed a short distance above the cage floor, lightly nudging the animal from sleep and encouraging low levels of activity until the animal maintains wakefulness on its own. The speed, direction, and duration of bar rotation are fully customizable.
Simulates gentle handling
• Limits unnecessary exercise
• Minimizes resources compared to manual deprivation
• Prevents sleep acclimation and habituation
• Accommodates water, food, and bedding
• Provides flexible scheduling controls
• Saves schedules for future use
• Integrates with synchronized video system
EFFECTIVENESS OF SLEEP DEPRIVATION IN MICE
The chart below shows a comparison of baseline sleep (purple bars) with a 24-hour sleep deprivation period (gold bars). Six mice (male, C57BL/6J) were maintained on a 12-hour lights-on (horizontal tan)/ 12-hour lights-off (horizontal black) schedule. EEG and EMG activity were recorded for 24 hours (baseline). During the following 24 hours, each animal was sleep deprived by programming the system to rotate the bar within ten seconds of the mouse entering a sleep-like state, as determined by active EEG feedback. Vertical bars represent sleep time in 2-hour bins
Tutorial Tip: Secure crossbar with teflon tape.