SLEEP DEPRIVATION SYSTEM by Pinnacle Technology is designed to gently restrict sleep without unnecessary exercise in rodents. This is achieved by a rotating bar placed a short distance above the cage floor, lightly nudging the animal from sleep and encouraging low levels of activity until the animal maintains wakefulness on its own. The speed, direction, and duration of bar rotation are fully customizable.
Pinnacle Technology’s Sleep Deprivation System offer biopotential recording systems for sleep, seizure and general research paradigms in freely moving mice and rats. The EEG/EMG systems use head-mounted amplification to produce exceptionally clean waveforms, even during animal movement. Wireless & tethered preconfigured three-channel systems are available. A flexible four-channel tethered system is available which includes the capability to incorporate simultaneous accelerometer, optogenetics or sensor measurements.
Simulates gentle handling
• Limits unnecessary exercise
• Minimizes resources compared to manual deprivation
• Prevents sleep acclimation and habituation
• Accommodates water, food, and bedding
• Provides flexible scheduling controls
• Saves schedules for future use
• Integrates with synchronized video system
EFFECTIVENESS OF SLEEP DEPRIVATION IN MICE
The chart below shows a comparison of baseline sleep (purple bars) with a 24-hour sleep deprivation period (gold bars). Six mice (male, C57BL/6J) were maintained on a 12-hour lights-on (horizontal tan)/ 12-hour lights-off (horizontal black) schedule. EEG and EMG activity were recorded for 24 hours (baseline). During the following 24 hours, each animal was sleep deprived by programming the system to rotate the bar within ten seconds of the mouse entering a sleep-like state, as determined by active EEG feedback. Vertical bars represent sleep time in 2-hour bins
Tutorial Tip: Secure crossbar with teflon tape.
See Pinnacle Technology’s full range of EEG/EMG systems here.
